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NanoDrop® ND-3300 Overview
The NanoDrop® ND-3300 Fluorospectrometer is based on the same patented sample retention technology (surface tension) utilized on the NanoDrop® ND-1000 Spectrophotometer.
UV excitation = 365 nm - filtered (<5% transmittance above 400nm)

Blue excitation = 470 nm

White excitation = 500-650 nm
Surface tension is used to hold a column of liquid sample in place while a measurement is made. This is done by pipeting the sample directly onto one measurement pedestal. A measurement column is then drawn between the ends of two optical fibers to establish the measurement path as shown below.
The measurement is made, typically in less than 10 seconds, and the spectrum and its analysis is shown on the screen of the attached PC and archived on the PC.

Once the measurement is complete, the sample is simply wiped from the measurement pedestals. The software enables individual user accounts for easy data segregation. The archived data can be manipulated by a spreadsheet program such as MS Excel.
Their emission maxima are listed below:
Technology in Brief
Technology in Brief ND-1000 Demo Video
ND-3300 Specifications:
Long Path Length

1mm

Sample Size Requirement

1-2 ul

Light Source

3 light-emitting diodes (LEDs) 

Detector

2048-element linear silicon CCD array

 
Wavelength Range

400-750 nm

 

Wavelength Accuracy

1 nm

 
Wavelength Resolution

8 nm (FWHM at Hg 546 m)

 

Measurement Cycle Time

10-15 seconds

 
Dimensions

20 cm X 15 cm x 12 cm

Sample Pedestal Material of Construction

303 Stainless Steel

 
Fiber Optic Cable Material of Construction

quartz

 
 
ND-3300 Performance Data
ND-3300 PC Requirements:
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Instrument Description
The uniquely clean optics of the patented retention system, combined with proprietary signal processing for the white LED applications, enables measurements across a wide range of wavelengths using sample volumes of 1 - 2 ul without cuvettes and costly filter changes.

The excitation source comes from one of three (3) solid-state light emitting diodes (LED's), which are oriented 90 ° to the detector.
Emitted light is collected using the classical 90 ° angle from the respective excitation source. A 2048-element CCD array detector, covering 400 - 750nm, is connected by optical fiber to the optical measurement surface. The spectrometer is configured with a cut filter to eliminate light transmission below 395 nm.
An added convenience is that no external power supply is required. All of the operating power is supplied by the USB port on the PC.