iPSCs can be induced by forcing somatic cells to express transcription factors associated with pluripotency eg Oct4, Klf4, Sox2, cMyc, etc (Takahashi et al., 2006). Many different methods have been successfully used to express different combinations of these pluripotency factors resulting in effectively reprogrammed iPSCs.
To keep your PSCs in a pluripotent state it is important to handle them with care. PSCs require strict culture media and passaging to keep them healthy and undifferentiated. The two culture systems commonly used for PSCs are either feeder-dependent or feeder-free culture systems.
ue to the pluripotent nature of PSCs they have the potential to differentiate into any cell type from neural cells to cardiomyocytes. This differentiation can be done simply using a cocktail of different biochemicals and growth factors.
BCR-ABL1 и ABL1 - QuantideX® qPCR BCR-ABL IS transcript analysis kit for monitoring chronic myeloid leukemia, Asuragen, USA.