Nextera XT DNA Library Preparation Kit
The Nextera XT DNA Library Preparation Kit offers a fast and easy workflow for preparing, quantifying, and normalizing libraries for sequencing small genomes, PCR amplicons, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA from a low amount of input DNA (1 ng), making this kit amenable for use with precious samples available in limited quantity.
- Provides rapid library preparation in as little as 90 min with only 15 min of hands-on time
- Eliminates library quantification before sample pooling and sequencing
- Delivers quick results in under 8 hr using Illumina sequencing systems
- Achieves high, even coverage and accurate calls for PCR amplicons
- Produces high-quality de novo assembly of small genomes
- Allows scalability by processing and uniquely barcoding up to 384 samples in a single experiment
Properties
Value
Properties
:Assay time
Value
:~5.5 hr from DNA extraction to normalized library. (Library prep time: ~90 minutes).
Properties
:Automation capability
Value
:Liquid handling robot(s)
Properties
:Hands-on time
Value
:15 min
Properties
:Input quantity
Value
:1 ng DNA
Properties
:Instruments
Value
:MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NextSeq 500 System
Properties
:Mechanism of action
Value
:Enzymatic fragmentation
Properties
:Method
Value
:Amplicon sequencing, 16s rRNA sequencing, De novo sequencing, Whole-genome sequencing, Shotgun sequencing
Properties
:Multiplexing
Value
:Up to 384 uniquely indexed samples may be pooled and sequenced together
Properties
:Nucleic acid type
Value
:DNA
Properties
:Specialized sample types
Value
:Not FFPE-compatible, Low-input samples, Single cells
Properties
:Species category
Value
:Compatible with any species
Properties
:Target insert size
Value
:300 bp–1.5 kb
Properties
:Technology
Value
:Sequencing
Properties
:Variant class
Value
:Single nucleotide polymorphisms (SNPs), Structural variants
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