Urinary Pathogen ID/AMR Enrichment Kit

The Urinary Pathogen ID/AMR Panel (UPIP) is a next-generation sequencing (NGS) research panel that enables detection and quantification of over 170 uropathogens responsible for urinary tract infections (UTIs) and co-infections and covers more than 3700 markers and their alleles associated with antimicrobial resistance (AMR). 

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Key features
  • Delivers simultaneous detection of 120+ bacteria, 35 viruses, 14 fungi, 4 parasites, and 27 sexually transmitted pathogen plus associated AMR alleles
  • Enriches uropathogen target regions from a range of host and environmental samples
  • Detects less common, challenging-to-grow, and frequently missed uropathogens
  • Outperforms shotgun metagenomics and conventional urine culture methods
Specifications

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Value

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Assay time

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< 9 hr library prep and enrichment time

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Automation capability

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Liquid handling robot(s)

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Hands-on time

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< 2 hr library prep and enrichment time

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Input quantity

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Based on volume (not concentration dependent)

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Instruments

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MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, MiSeq i100 System, MiSeq i100 Plus System

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Mechanism of action

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On-bead tagmentation followed by a single hybridization step

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Method

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Targeted DNA sequencing, Target enrichment

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Multiplexing

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Up to 384 samples in a single run with unique dual indexes

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Nucleic acid type

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DNA

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Sample type details

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DNA extracted from human urinary clinical research samples and other surveillance related samples such as wastewater

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Specialized sample types

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Not FFPE-compatible

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Species category

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Other, Human, Virus, Bacteria

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Technology

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Sequencing

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