NovaSeq 6000 System

The NovaSeq 6000 System providing users with the throughput, speed, and flexibility to complete projects faster and more economically than ever before. Applications requiring large amounts of data, such as human wholegenome sequencing (WGS), ultradeep exome sequencing, and tumor–normal profiling can now be completed in a more cost-effective manner. The same instrument can be used for less data-intensive methods, such as targeted resequencing.

The NovaSeq 6000 System offers tremendous flexibility in sequencing options, supporting an extensive range of applications. Users can mix and match between three flow cell types (S1, S2, or S4), run one or two flow cells at a time, and choose between multiple read lengths to easily adjust output and sample throughput for each sequencing run. For faster sample loading, the NovaSeq 6000 System offers the standard workflow featuring fully automated onboard cluster generation for ease of use and reduced hands-on time.

The NovaSeq 6000 System expands NGS possibilities for all researchers. With unmatched scalable throughput, tremendous flexibility for a range of applications, and streamlined operation, the NovaSeq 6000 System is the most powerful high-throughput Illumina sequencing system to date, perfectly positioned to help users uncover more about the genome than ever before.

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Catalog number
NovaSeq 6000 System
Properties Value
Large Whole-Genome Sequencing (human, plant, animal) +
Small Whole-Genome Sequencing (microbe, virus) +
Exome Sequencing +
Targeted Gene Sequencing +
Transcriptome Sequencing +
Gene Expression Profiling with mRNA-Seq +
Targeted Gene Expression Profiling -
Long-Range Amplicon Sequencing* --
miRNA & Small RNA Analysis +
DNA-Protein Interaction Analysis +
Methylation Sequencing +
16S Metagenomic Sequencing --
Run Time (hours) 13-44
Maximum Output 134-3000 Gb
Maximum Reads Per Run (millions) 450-20 bln.
Maximum Read Length (bp) 2 × 250
Small Whole-Genome Sequencing (microbe, virus) --
Chromatin Analysis (ATAC-Seq, ChIP-Seq) +
Metagenomic Profiling (shotgun metagenomics, metatranscriptomics)(shotgun metagenomics, metatranscriptomics) +
Single-Cell Profiling (scRNA-Seq, scDNA-Seq, oligo tagging assays) +
Cell-Free Sequencing & Liquid Biopsy Analysis +
Mitochondrial DNA Analysis --