Respiratory Pathogen ID/AMR Enrichment Panel Kit

The Respiratory Pathogen ID/AMR Enrichment Panel (RPIP) Kit is a next-generation sequencing (NGS)-based respiratory pathogen panel and library prep and enrichment kit that targets > 280 respiratory pathogens and > 2000 antimicrobial resistance (AMR) markers in a single assay, providing both pathogen identification and associated AMR information.

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Key features
  • Comprehensive targeting of DNA and RNA respiratory pathogens and associated AMR markers/alleles that provide known predicted resistance to antimicrobial agents
  • Robust and sensitive detection of low-abundance respiratory pathogen and antimicrobial resistance alleles using various sequencing read lengths and depths
  • Sensitive detection of single or multiple targeted pathogens in low or high complexity samples
  • Powerful pathogen quantitation using 10 commercially available spike-in controls
Specifications

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Value

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Assay time

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< 9 hr library prep time

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Automation capability

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Liquid handling robot(s)

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Hands-on time

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< 2 hr library prep time

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Input quantity

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Based on volume (not concentration dependent)

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Instruments

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MiSeq System, NextSeq 550 System, MiniSeq System, MiSeq i100 Plus System, MiSeq i100 System

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Mechanism of action

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On-bead tagmentation followed by a single hybridization step

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Method

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Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment

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Multiplexing

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Up to 384 samples in a single run with unique dual indexes

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Nucleic acid type

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DNA, RNA

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Species category

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Fungal, Virus, Bacteria

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Technology

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Sequencing

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